University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Food -> chewing gum

Citations 1

"Flow Injection Determination Of D-mannitol With Immobilized Mannitol Dehydrogenase"
Anal. Chim. Acta 1991 Volume 244, Issue 1 Pages 105-107
Nobutoshi Kiba, Yukari Inoue and Motohisa Furusawa

Abstract: In the flow injection method described (diagram of apparatus given), 5 mM NAD+ was mixed with 0.2 M carbonate buffer of pH 10.0 (total flow-rate 0.5 mL min-1) and passed through a mixing coil (1 m x 0.5 mm). Sample solution (50 µL) was injected into the stream, the mixture was passed through a column reactor (5 cm x 4 mm) containing mannitol dehydrogenase immobilized on poly(vinyl alcohol) beads (cf., Ibid., 1991, 243, 183) at 37°C and the NADH produced was detected fluorimetrically at 465 nm (excitation at 340 nm). The calibration graph was rectilinear for 0.5 to 100 µM-D-mannitol and the detection limit was 0.1 µM. The sampling rate was 30 h-1 and the coefficient of variation (n = 12) was 1%. The method was applied in the analysis of celery juice and chewing gum (prep. described); results compared well with those by a chromatographic method. The method should also be applicable to biological samples.
d-Mannitol Fluorescence Buffer Column Immobilized enzyme