University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Environmental -> soil -> sand

Citations 2

"Flow Injection Analysis Of C-fuel Oil-contaminated Samples Based On The Fluorescence Detection Of Polycyclic Aromatic Hydrocarbons"
Anal. Sci. 1998 Volume 14, Issue 4 Pages 845-847
Akiko UTSUMI, Atsuko NAKASHIMA, Kyoko ANDO, Ryoichi KIZU and Kazuichi HAYAKAWA

Abstract: On Jan. 2, 1997, >6,000 kL of C-fuel, a fuel oil, was spilled in the Sea of Japan and drifted toward 8 Prefectural sea coasts from Shimane to Yamagata. Polycyclic arom. hydrocarbons were determined in the spilled oil, sea sand, and water using HPLC equipped with a fluorescence detector. Although the HPLC method was accurate and quant., it takes too much time for rapid anal. of many oil-polluted environmental samples. Thus, a flow injection analysis method was used as a rapid screening test of C-fuel oil pollution. Results using this method to analyze sea sand polluted with oil from this spill are also reported.
Polycyclic aromatic hydrocarbons Fluorescence

"Development And Application Of An Automated Quasi-continuous Immunoflow-injection System To The Analysis Of Pesticide Residues In Water And Soil"
J. Agric. Food Chem. 1994 Volume 42, Issue 4 Pages 1041-1047
Christine Wittmann and Rolf D. Schmid

Abstract: Sample was pumped for 3 min at 0.78 ml/min through a reactor containing polyclonal or monoclonal anti-atrazine antibodies immobilized on glass or polystyrene beads via the avidin-biotin system (cf. Locascio-Brown et al, Anal Chem., 1990, 62, 2587). Then 40 µL of peroxidase-labelled atrazine was injected into PBS of pH 7.2 and pumped through the column in a 'stop-go' cycle in five intervals of 20 s. The column was rinsed with carrier buffer for 2 min and then 40 µL each of 5 mM 3-(p-hydroxyphenyl)propionic acid and 2 mM H2O2 were injected into PBS carrier stream and carried on to the reactor whereupon the flow was stopped for 2 min. The flow was restored and the fluorescence of the enzymatic reaction product was measured at 404 nm (excitation at 320 nm). The column was regenerated by washing with 10 mM glycine/HCl buffer of pH 2 for 1.5 min at 0.72 ml/min and then with carrier buffer for 1.5 min. Detection limits were 1 and 30 ng/l with polyclonal and monoclonal antibodies, respectively. For 0.1-2 µg/l of atrazine in water the RSD (n = 4) were 2.9-8.3%. Results agreed with those obtained by GC. Recoveries of atrazine from sand soil and clay soil were 100 and 88%, respectively.
Atrazine Immunoassay Fluorescence GC Polystyrene beads Immobilized antibody