University of North Florida
Browse the Citations
-OR-

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

View Stuart Chalk's profile on LinkedIn

Commercial product

Classification: Commercial product -> shampoo

Citations 2

"Analysis Of Amphoteric Surfactants By Liquid Chromatography With Post-column Detection. 2. Imidazoline-type Amphoteric Surfactants Derived From Sodium Chloroacetate"
J. Chromatogr. A 1983 Volume 267, Issue 1 Pages 133-148
Jiro Kawase and Kazuro Tsuji, Yutaka Yasuda and Kazuaki Yashima

Abstract: Such surfactants were separated by HPLC on columns (15 cm x 4.6 mm, analytical; or 25 cm x 8 mm, semi-preparative) of Develosil ODS-3, with aqueous 45% acetonitrile containing 0.2 M NaClO4 (pH 2.5) as mobile phase; detection was at 210 or 230 nm, or after post-column derivatization as described in Part I (Ibid., 1983, 264, 415).The method yielded information on the alkyl-chain distribution of the original fatty acids, the degree of N-substitution by the electrophilic reactant, the reaction mechanism and the conditions under which the surfactants were prepared.The method was applied in analysis of a shampoo.
Surfactants, amphoteric HPLC Spectrophotometry Post-column derivatization

"Determination Of Alkanolamines By High Performance Liquid Chromatography With Post-column Derivatization"
Bunseki Kagaku 1981 Volume 30, Issue 6 Pages 353-357
Atsuo NAKAE, Kenji MANSHO, Kazuro TSUJI

Abstract: Mono-(MEA), di-(DEA), tri-(TEA), N-methyl-(Methyl EA), and N, N-diethylethanolamine (Diethyl EA) were separated and determined by high speed ion-exchange chromatography with a post-column derivatization method. The capacity factor of alkanolamines was affected by pH and sodium ion concentration of the eluent. The post-column derivatization for the detection of alkanolamines in the column effluent involves the chlorination of alkanolamines reacting with hypochlorite to form chloramine derivatives, followed by the selective destruction of the excess hypochlorite by nitrite and the subsequent reaction of chloramine derivatives with iodide. The resulting triiodide ion is measured spectrophotometrically at 350 nm. Raising pH of the hypochlorite solution and the reaction bath temperature for chlorination, the peak area of MEA having primary amino group decreased, that of DEA and Methyl EA having secondary amino group was approximately constant, and that of TEA and Diethyl EA having tertiary amino group increased. Therefore, pH of the hypochlorite solution was adjusted at 6.5 (phosphate buffer) and the reaction bath temperature at 65°C. The calibration curves for alkanolamines were linear within a range of 1.5 - 30 nmol. The satisfactory result for precision and recovery was obtained by analysis of the solution containing known amounts of alkanolamines. The proposed method does not require any pretreatment of samples, because it involves both the separation and the selective detection steps. Alkanolamines in the commercial shampoos were determined directly without any interference.
Alkanolamines HPLC Post-column derivatization