University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Commercial product -> dental -> anaesthetic

Citations 1

"Liquid Chromatographic Determination Of Felypressin Using A Column-switching Technique And Post-column Derivatization"
J. Chromatogr. A 1990 Volume 521, Issue 1 Pages 141-147
Mats Svensson* and Kerstin Gröningsson

Abstract: The nonapeptide felypressin (I) was determined in a dental anaesthetic containing prilocaine hydrochloride after cartridge cleanup. The cartridge (25 mm x 4 mm) packed with Superspher 60 RP-8 (4 µm; Merck) was connected in series with the analytical column (5 cm x 4 mm) of Superspher 60 RP-8e (4 µm). During cleanup (14 min) the mobile phase (1 mL min-1) of phosphate buffer (pH 6.0) - acetonitrile (22:3) was fed through the cartridge only, then flow was switched through cartridge and column and solvent component ratio was changed to 4:1 for the analytical phase (9 min). Post-column derivatization was by addition of 0.03% (w/v) of fluorescamine and 0.1% (v/v) of Brij 35 in acetonitrile (0.25 mL min-1) and fluorimetric detection was at 470 nm (excitation at 390 nm). For determination of I in Citanest Octapressin injection solution, coefficient of variation were 1.7% and mean recovery was 101.8% (n = 6). Life of the cleanup cartridge was ~100 determinations.
Felypressin LC Fluorescence Post-column derivatization Column Buffer pH