University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Cell -> ovary -> hamster

Citations 1

"Flow Injection Microscopy As A Tool For Two-parameter Monitoring Of Cellular Responses"
Fresenius J. Anal. Chem. 1998 Volume 362, Issue 1 Pages 67-72
Ilkka Lähdesmäki and J. Ruzicka

Abstract: Flow injection fluorescence microscopy (FIFM) was further developed to become a useful tool for a multiparametric study of the response of living cells to a biochemical stimulus. Rather than recording several parameters simultaneously, the multiparametric measurements are carried out in a sequential fashion: internal Ca concentration. and pH of Chinese hamster ovary cells were alternately monitored in subsequent runs where the cells were exposed to carbachol. Delivery of carbachol onto the cells was carried out by the flow injection app. in the FIFM setup. Fluorescent indicators fura-2 and BCECF were loaded into the cells to provide the Ca- and pH-dependent signals, respectively. The fluorescent signals were detected by the fluorescent microscope domain of the FIFM setup. Comparison of the temporal dependence of the response curves shows differences in the 2 responses. The experimental evidence is supported by a novel functional tracer test that is used to characterize the kinetics of cell-stimulation interaction.
Calcium pH Microscopy Kinetic