University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Cell -> lymphocyte -> human

Citations 1

"Fast Kinetic Measurements And Online Dilution By Flow Injection Cytometry"
Cytometry 1994 Volume 16, Issue 4 Pages 324-330
Walter Lindberg, Louis D. Scampavia, Jaromir Ruzicka, Gary D. Christian

Abstract: An improved flow injection cytometry (FIC) system suitable for fast cellular kinetic measurements and online dilution is described. The instrument allows for measurements from 1.2 s (± 0.05 s) after the initiation of mixing, up to any time thereafter. Crucial factors in determining fast kinetic measurements, such as the displacement volume for sample introduction, rate of transport to cytometric interrogation point, and the mixing speed are evaluated and discussed. By varying the volume aspirated, this instrument can facilitate the dilution of cells and/or reagent over a range of one order of magnitude. The fast kinetic and online dilution capabilities were demonstrated by online staining of DNA in trout erythrocytes with 4',6-diamidino-2-phenylindole (DAPI). The utility of the instrument for measurement of enzyme kinetics was illustrated using human lymphocytes, measuring the glutathione S- transferase (GST) catalyzed reaction between monochlorobimane (MCB) and glutathione (GSH).
Enzyme, glutathione S-transferase Flow cytometry Kinetic