University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Cell -> leucocyte

Citations 1

"High-sampling-rate Automated Continuous-flow Fluorimetric Technique For Analysis Of Nanogram Levels Of Histamine In Biological Samples"
Anal. Biochem. 1983 Volume 133, Issue 1 Pages 16-29
B. Lebel

Abstract: The method is based on that of Shore et al. (J. Pharmacol. Exp. Ther., 1959, 127, 182), automated to allow 60 analyzes in 1 h. Flow schemes, one with and the other without dialysis, are presented. Histamine is caused to react with phthalaldehyde, and the fluorescence of the product is measured at 450 nm (excitation at 360 nm); peak heights and baseline corrections are calculated by a computerized system. The limit of detection is 25 pg, and the rectilinear calibration range is 0 to 5 µg mL-1 of histamine. The method has been applied to whole blood, tissues and cell cultures.
Histamine Fluorescence Dialysis