University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Rat

Classification: Cell -> erythrocyte -> rat

Citations 1

"Determination Of Thiamine And Its Phosphate Esters In Human And Rat Blood By High Performance Liquid Chromatography With Post-column Derivatization"
J. Chromatogr. A 1985 Volume 332, Issue 1 Pages 181-188
Mieko Kimura and Yoshinori Itokawa

Abstract: Blood, erythrocytes and plasma were deproteinized with aqueous 10% trichloroacetic acid. After centrifugation, the supernatant solution were analyzed by HPLC on a µBondapak C18 column (25 cm x 4 mm) with 0.2 M NaH2PO4 in aqueous 0.3% acetonitrile as the mobile phase (1.0 mL min-1); the eluates were subjected to post-column derivatization with 0.1% of K3Fe(CN)6 in aqueous 15% NaOH and spectrofluorimetric detection at 450 nm (excitation at 375 nm). The detection limit for thiamine and its phosphate esters was 30 fmol and recoveries were 95.0 to 100.2%. The calibration graphs were rectilinear for 50 to 200 fmol.
Thiamine Thiamine triphosphate Thiamine monophosphate HPLC Fluorescence Post-column derivatization