University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Cell -> cultures

Citations 1

"Chemiluminometric Enzyme Sensors For Flow Injection Analysis"
Anal. Chim. Acta 1995 Volume 303, Issue 1 Pages 109-120
U. Spohn*, F. Preuschoff, G. Blankenstein, D. Janasek, M. R. Kula and A. Hacker

Abstract: Enzyme sensors for H2O2 or, for xanthine or hypoxanthine were prepared by covalent immobilization of microbial peroxidase (from Athromyces ramosus) or xanthine oxidase, respectively, on pre-activated nylon membranes and these were mounted in a 7.5 µL flow cell. Chemiluminescent procedures were developed for the cited analytes, based on the luminol-H2O2 reaction, wherein the luminescence intensity was measured by use of either a fiber-optic bundle connected to a photomultiplier, or a photodiode. With use of the fiber-optic detection system detection limits for H2O2, hypoxanthine and hypoxanthine were 0.1, 2 and 5 µM, respectively. Bienzyme sensors were also developed for glutamate, lysine and xanthine by co-immobilization of microbial peroxidase with the corresponding analyte oxidase on the sensor membrane. Use of the bienzyme sensors permitted the determination of the cited analytes in the range 1 µM to 1 mM. Finally, a system is described in which the membrane sensor with immobilized peroxidase was used in combination with parallel packed bed enzyme reactors for the sequential determination of glucose, lactate, glutamate, glutamine and NH3 in animal cell cultures.
Glutamate Glutamine Hydrogen peroxide Hypoxanthine Lysine Xanthine Chemiluminescence Sensor Optical fiber Immobilized enzyme Nylon Reactor Photodiode