University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Cell -> acremonium chrysogenum

Citations 1

"Application Of Biosensors With An Electrolyte Isolator Semiconductor Capacitor (EIS-CAP) Transducer For Process Monitoring"
Process Biochem. 1998 Volume 33, Issue 2 Pages 175-180
C. Menzel, T. Lerch, K. Schneider, R. Weidemann, C. Tollnick, G. Kretzmer, T. Scheper and K. Schüger*

Abstract: A fluoride sensitive (pF), buffer capacity insensitive electrolyte isolator semiconductor capacitor (pF-EIS-CAP) chip was used as a transducer for biosensors with co-immobilized enzymes. Hydrogen peroxide forms fluoride ions in stoichiometric amounts in the presence of p-fluoraniline and peroxidase (POD). This was measured by the pF-EIS-CAP-sensor. The combination of this sensor with various oxidases allows the measurement of the concentration. of several analytes. Sugar syrup (glucose, maltose, maltotriose and other oligosaccharide) concentrations. were analyzed with co-immobilized β-amylase, amyloglucosidase, mutarotase, glucose oxidase and peroxidase. The glucose concentration. was analyzed with co-immobilized glucose oxidase and peroxidase, the ethanol concentration. with co-immobilized alcohol oxidase and peroxidase and the phosphate concentration. by co-immobilized nucleoside phosphorylase, xanthine oxidase and peroxidase. These (Bio-pF-EIS-CAP) sensors were integrated in flow injection analysis systems. The medium components were monitored online during Saccharomyces cerevisiae, Acremonium chrysogenum and recombinant baby hamster kidney (rBHK) cell cultivations. The agreement between the off- and online concentrations. of the medium components was within 10%.
Hydrogen peroxide Glucose Maltose Maltotriose Oligosaccharides Sensor Sensor Method comparison