University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Thymus

Classification: Biological tissue -> thymus -> mouse

Citations 1

"Determination Of O-phosphoethanolamine In Male And Female Mouse Tissues By High Performance Liquid Chromatography"
J. Pharm. Soc. Jpn. 1986 Volume 106, Issue 8 Pages 694-697
YUKITOSHI HAYASE, MIHO MURAKAMI, YOSHINARI TAKAGI

Abstract: Phosphoethanolamine was extracted from mouse tissues (e.g., brain, salivary gland, thymus, liver, spleen and kidney) with trichloroacetic acid before determination by cation-exchange chromatography on a column (15 cm x 4 mm) of Shimadzu ISC-07/S at 55°C with, as mobile phase, 0.2 M Na citrate - HClO4 buffer (pH 2.0) containing 7% of ethanol and 0.01% of octanoic acid at 0.3 mL min-1. Fluorimetric detection was used with post-column derivatization with phthalaldehyde. Recovery was 93.8 to 103.8%.
o-Phosphoethanolamine HPIC Fluorescence Heated reaction Post-column derivatization