University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> stomach -> rat -> simulated

Citations 1

"Aluminum Fractionation In A Simulated Rat Stomach: An In Vitro Study"
Analyst 1995 Volume 120, Issue 3 Pages 713-720
Lars-Göran Danielsson, Anders Sparén and Anders Wicklund Glynn

Abstract: The conditions in a rat stomach were simulated using an in vitro system and the effects of various parameters such as pH, feed content and the presence of enzymes on the solubility and distribution of Al species were studied. Experiments were performed at 37°C using ground rat feed, at a fixed pH and with hog pepsin A as the enzyme. Al (4 mg/l) was added and the mixture was stirred for 2 h. After centrifugation, the supernatant solution was filtered and analyzed for Al species. Quickly-reacting Al was determined by flow injection spectrophotometry by a modification of the method described by Clarke et al. (Int. J. Environ. Anal. Chem., 1992, 48, 77). Total dissolved Al, Fe and P were determined by ICP-AES at 308.2, 238.2 and 214.9 nm, respectively. Possible reasons for the observed distribution of the Al species are discussed. The pattern of the in vitro results was confirmed by measurements in pooled rat stomachs from in vivo experiments. The results highlighted the importance of fractionation measurements in bioavailability studies of Al.
Aluminum Spectrophotometry Sample preparation 8-Hydroxyquinoline Complexation Extraction In vitro monitoring