University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> pancreas

Citations 2

"Determination Of Online Differential Refractive Index And Molecular Weight Via Gradient HPLC Interfaced With Low-angle Laser Light Scattering, Ultraviolet, And Refractive Index Detection"
Anal. Chem. 1993 Volume 65, Issue 3 Pages 283-286
Rohin Mhatre and Ira S. Krull

Abstract: Pancreatic ribonuclease, trypsinogen and α-chymotrypsinogen were dissolved in 5 mM bis-Tris/0.2 M dextrose (buffer A), to give solution of 2.5-3.5 mg/ml and applied to a column (10 cm x 4.6 mm) of strong cation-exchange polymer-based packing operated with gradient elution (0.5 ml/min) with buffer A and 5 mM bis-Tris/0.5 M NaCl (buffer B) from 0-80% buffer B over 20 min with online detection using a low-angle laser light scattering photometer, a UV-visible detector at 280 nm and a laser-based differential refractive index detector at 670 nm connected in series with a PC for data collection and processing. The system was also used in the FIA mode to measure the molar absorptivities of the proteins. Off-line refractive index measurements were performed with a laser-based differential refractometer, at 633 nm. Online differential refractive index measurements were similar to those determined off-line and required less sample (3-4 mg as opposed to 200 mg). Molecular wt. determinations agreed with literature values to within 3% and protein recoveries ranged from 66-87%. Other biopolymers, such as DNA fragments can be characterized using the same conditions.
Ribonucleosides Nucleic acids, deoxyribo HPLC Spectrophotometry Refractive index

"Inductively Coupled Plasma Atomic Emission Spectrometric Determination Of Copper By Suction-flow Online Liquid -liquid Extraction Of Its Macrocyclic Dioxotetramine Chelate"
Bull. Chem. Soc. Jpn. 1987 Volume 60, Issue 5 Pages 1930-1932
Takahiro Kumamaru,Yoko Nitta,Hiroshi Matsuo and Eiichi Kimura

Abstract: Sample solution, containing ~30 g mL-1 of Ni(II), was placed in a PTFE suction cup and mixed with 5 mM 6-hexadecyl-1,4,8,11-tetra-azacyclotetradecane-5,7-dione and 1 M borate buffer (pH 9). The solution was pumped to a segmentor containing CHCl3 and the segmented solution was carried to an extraction coil and a phase separator. The organic extract was pumped into the nebulizer of the ICP spectrometer and Cu(II) was determined at 324.754 nm. Response was rectilinear for up to 500 ng mL-1 of Cu(II) and the detection limit was 1.5 ng mL-1. The coefficient of variation (n = 10) was 2.2%. Aluminium, Cr(III) and Fe(III) interfered. The method was applied to determine Cu(II) in biological tissues, and results agreed well with those obtained by graphite-furnace AAS.
Copper Spectrophotometry Sample preparation Chelation Extraction