University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> muscle -> rat

Citations 1

"Determination Of Vitamin B6 In Foods And Other Biological Materials By Paired-ion High Performance Liquid Chromatography"
J. Agric. Food Chem. 1985 Volume 33, Issue 3 Pages 359-363
Jesse F. Gregory and Debra Feldstein

Abstract: Food, human plasma or milk, or rat liver or muscle samples, were homogenized in sulfosalicylic acid with 4'-deoxypyridoxine (as internal standard) and CH2Cl2. After centrifugation, the aqueous phase was applied to a column of Bio-Rad AG2-X8 anion-exchange resin (200 to 400 mesh; Cl- form) with 0.1 M HCl as mobile phase. The fraction containing pyridoxine was subjected to HPLC on a column (3 cm x 4.6 mm) of octadecylsilica (3 µm) with gradient elution with 0 to 2.5% of propan-2-ol in 33 mM potassium phosphate - 8 mM octanesulfonic acid (pH 2.2). Post-column derivatization was with NaHSO3 in 1 M sodium phosphate buffer (pH 7.5) and fluorescence detection was at 400 nm (excitation at 330 nm). The coefficient of variation were <2%; recoveries were 78.7 to 103.2%. Good correlation with published data was achieved.
Vitamin B6 Pyridoxine HPLC Ion exchange Fluorescence Post-column derivatization