University of North Florida
Browse the Citations

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

View Stuart Chalk's profile on LinkedIn


Classification: Biological tissue -> muscle -> pig

Citations 2

"Determination Of Residues Of Carbadox And Some Of Its Metabolites In Swine Tissues By High Performance Liquid Chromatography Using Online Pre-column Enrichment And Post-column Derivatization With UV - Visible Detection"
J. Chromatogr. A 1988 Volume 456, Issue 1 Pages 105-119
M. M. L. Aerts*, W. M. J. Beek and H. J. Keukens, U. A. Th. Brinkman

Abstract: Homogenized muscle, liver or kidney was extracted with methanol - acetonitrile (1:1) and the extract was cleaned up on a column (40 cm x 10 mm) of alumina - Florisil. The percolate was evaporated under N, the residue was dissolved in water and the solution was extracted with 2,2,4-trimethylpentane. Portions (2 ml) of the aqueous phase were injected into a column-switching HPLC system comprising an analytical column (20 cm x 3 mm) of ChromSpher C18 (5 µm) with a guard column (1 cm x 2.1 mm) and a preliminary enrichment column (6 cm x 4.6 mm), both of Bondapak C18/Corasil (37 to 50 µm). The mobile phase was acetonitrile - 0.01 M Na acetate buffer of pH 6 (3:17). Post-column derivatization with 0.5 M NaOH was studied. Flow rates (ml min-1) were 0.6 (eluent), 0.5 (sample enrichment) and 0.23 (derivatization reagent). Detection was at 420 nm. Limits of determination were 1 to 5 µg kg-1, recoveries were 81 to 87% and the coefficient of variation was 4 to 10% (n = 8 to 10).
Carbadox HPLC Spectrophotometry Post-column derivatization

"Determination Of Ionophores In The Tissues Of Food Animals By Liquid Chromatography"
Food Addit. Contam. 1995 Volume 12, Issue 6 Pages 731-737
Gerhardt G, Salisbury CD, Campbell HM

Abstract: A liquid chromatographic method for determining residues of ionophores in bovine, porcine, and avian tissues is described. Tissues were extracted with iso-octane-ethyl acetate and the extracts purified on silica solid-phase extraction columns. Monensin, narasin and salinomycin were detected by UV absorbance following post-column derivatization with vanillin, and lasalocid was detected underivatized using fluorescence. In muscle, kidney and fat, all drugs were determined from a single sample extract. In liver, a separate extraction was done to determine lasalocid. The detection limit for lasalocid, narasin and salinomycin was 5 ppb; for monensin it was 2 ppb. Average recoveries were: lasalocid-71.0%; monensin-94.3%; salinomycin-97.2%; narasin-94.1%.
Lasalocid Monensin Narasin Salinomycin HPLC Fluorescence Post-column derivatization