University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> muscle -> herring

Citations 1

"Simultaneous Determination Of Biogenic Amines By Reversed-phase High Performance Liquid Chromatography"
J. Chromatogr. A 1990 Volume 508, Issue 1 Pages 225-228
Satoru Suzuki, Kentaro Kobayashi, Junko Noda, Tetsuya Suzuki and Kozo Takama

Abstract: The method of Gamoh and Fujita (Shimadzu Rev., 1987, 44, 237) was modified for the determination of histamine and five other biogenic amines in herring muscle. Dried herring (5 g) was homogenised with 10% trichloroacetate acid (2 x 20 ml), centrifuged at 550 g for 20 min, and the combined extracts were diluted to 50 mL. A portion (5 µL) of the solution was analyzed by HPLC on a column (15 cm x 6 mm) of Shim-Pak CLC-ODS operated at 50°C with gradient elution (1.1 mL min-1) with (i) 0.01 M Na hexanesulfonate in 0.1 M NaClO4 (pH 4.0) and (ii) a mixture of (i) - methanol (1:3) adjusted to pH 3.0 (details given). After post-column o-phthalaldehyde derivatization fluorimetric detection was at 455 nm (excitation at 345 nm). The method can also be applied in clinical analyzes.
Amines, biogenic Histamine Clinical analysis HPLC Fluorescence Sample preparation Simultaneous analysis Extraction Column pH Post-column derivatization