University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> lung -> rat

Citations 1

"Method For The Sensitive Analysis Of Individual Molecular Species Of Phosphatidylcholine By High Performance Liquid Chromatography Using Post-column Fluorescence Detection"
J. Chromatogr. B 1987 Volume 415, Issue 1 Pages 241-251
Anthony D. Postle

Abstract: Total phosphatidylcholine(I) in CHCl3 extracts of rat liver and lung was isolated from other phospholipid classes by HPLC on a column (30 cm x 4.9 mm) of µPorasil (10 µm) with hexane - propan-2-ol - water (60:80:9) as mobile phase (1 mL min-1) and detection at 200 nm. The eluate containing I was evaporated and the residue was dissolved in trifluoroethanol for analysis of individual molecular species by reversed-phase HPLC. Columns (25 cm x 4.6 mm) of µBondapak C18 (10 µm), Spherisorb ODS 1 and ODS 2 (5 µm), and Apex ODS 1 and ODS 2 (5 µm) were evaluated with mobile phases (1 mL min-1) of 40 mM choline chloride in methanol - water - acetonitrile. Initial detection was at 200 nm followed by post-column derivatization at 50°C with 1,6-diphenylhexa-1,3,5-triene and fluorescence detection at 460 nm (excitation at 340 nm). The between-run coefficient of variation (n = 10) were 0.32 to 0.68%. The stationary phase Apex ODS 2 gave the most efficient separation, and both saturated and unsaturated species could be determined.
Phosphatidylcholine HPLC Fluorescence Heated reaction Post-column derivatization