University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> lung -> rabbit

Citations 1

"High Performance Liquid Chromatography Post-column Derivatization With Fluorescence Detection To Study The Influence Of Ambroxol On Dipalmitoylphosphatidylcholine Levels In Rabbit Eustachian Tube Washings"
J. Chromatogr. A 1991 Volume 553, Issue 1 Pages 1-6
M. Kitsos, C. Gandini, G. Massolini and E. De Lorenzi, G. Caccialanza

Abstract: Eustachian tube or lung washings (10 ml) were centrifuged and the supernatant solution was freeze-dried before CHCl3 (5 ml) was added. After filtration, the solution was dried under N and the residue was dissolved in methanol (500 µL). A 100 µL portion was analyzed by HPLC on a column (10 cm x 4.6 mm) of ODS Hypersil (5 µm) with isocratic elution with 40 µM-choline chloride in methanol and THF (1 mL min-1). Fluorescence detection was at 460 nm (excitation at 340 nm) with use of 1,6-diphenyl-1,3,5-hexatriene in THF and 0.001% of polysorbate 20 (1.2 mL min-1). Levels of dipalmitoylphosphatidylcholine were also determined after ambroxal chloride administration.
dipalmitoylphosphatidylcholine HPLC Fluorescence Column Filtration Post-column derivatization