University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> lung -> human

Citations 1

"Sensitive Quantitation Of Chromium-DNA Adducts By Inductively Coupled Plasma Mass Spectrometry With A Direct Injection High-efficiency Nebulizer"
Toxicol. Sci. 1998 Volume 46, Issue 2 Pages 260-265
Jatinder Singha, John A. McLean, Daryl E. Pritchard, Akbar Montaser, and Steven R. Patierno

Abstract: A novel method is described for the sensitive detection of Cr-DNA adducts. Cr-DNA adducts were determined in 1 µg DNA from normal human lung fibroblasts exposed to sodium chromate using microscale flow injection analysis with a direct injection high-efficiency nebulizer and inductively coupled plasma mass spectrometry detection. The frequency of Cr-DNA adducts increased in a dose-dependent sigmoidal manner, indicating satn. and toxicity. The low detection limits (on the order of parts per trillion) allows the detection of as few as 2 Cr adducts per 10,000 bases, which, coupled with the small DNA sample requirement, makes this technique suitable for measuring metal-DNA adducts as biomarkers of exposure to toxic and carcinogenic metals such as Cr, in cultured cells, animals, and humans. (c) 1998 Society of Toxicology.
Chromium, adducts, DNA Mass spectrometry Interface Nebulizer