University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> lung

Citations 1

"Picomole Analysis Of Glutathione, Glutathione Disulfide, Glutathione S-sulfonate And Cysteine S-sulfonate By High Performance Liquid Chromatography"
Anal. Biochem. 1985 Volume 151, Issue 2 Pages 418-423
Douglas A. Keller and Daniel B. Menzel

Abstract: Glutathione(I), glutathione disulfide(II), glutathione S-sulfonate(III) and cysteine S-sulfonate(IV) were separated by HPLC on a column of Spherisorb 5 SAX strong anion exchanger with non-linear gradient elution with 0 to 30% of 144 mM Li citrate - 1 mM EDTA (pH 5.3) in 5 mM citric acid - 1 mM EDTA; determination was by post-column derivatization with phthalaldehyde and 2-mercaptoethanol at 70°C, and fluorescence detection at 430 to 470 nm (excitation at 305 to 395 nm). The detection limits were 10, 10, 50 and 200 pmol for I, II, III and IV, respectively; the respective recoveries were 102.6, 103.3, 95.5 and 99.7%. The method was applied to the analysis of supernatant solution obtained after homogenization of lung tissue with HClO4.
Glutathione Glutathione disulfide Glutathione S sulfonate S-sulfonatecysteine HPLC Fluorescence Heated reaction Post-column derivatization