University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> liver -> duck

Citations 1

"Determination Of N-methylcarbamate Pesticides In Liver By Liquid Chromatography"
J. AOAC Int. 1989 Volume 72, Issue 4 Pages 586-592
Ali MS

Abstract: A 21-g portion of bovine, porcine or duck liver was extracted with 200 mL of CH2Cl2 and the extract was dried (Na2SO4). After evaporation to 1 to 2 ml, the residue was dissolved in CH2Cl2 - cyclohexane (1:1; 7.5 ml) and cleaned up by gel-permeation chromatography on a column (60 x 2.5 cm) containing 60 g of BioBeads SX-3 resin (200 to 400 mesh), with CH2Cl2 - cyclohexane (1:1) as mobile phase (5.0 mL min-1). The eluate was evaporated and the residue was dissolved in CH2Cl2 and further cleaned up on an Aminopropyl Bond Elut cartridge, before transfer of residue to methanol and HPLC. Analysis was on a column (25 cm x 4.6 mm) of Zorbax C-8 (5 µm), with aqueous 12 to 70% acetonitrile over 30 min, then over 1 min to aqueous 80% acetonitrile (held for 8 min) as mobile phase (1.5 mL min-1). Post-column derivatization with phthalaldehyde was followed by fluorescence detection of 10 carbamate derivatives at 418 nm (excitation at 340 nm). Recovery was >80% with coefficient of variation of 17%; detection limits were 5 to 10 ppb.
Carbamates, N-methyl HPLC Fluorescence Sample preparation Post-column derivatization