University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> liver -> chicken

Citations 1

"Determination Of Total Phosphorus In Biological Samples By Flow Injection Analysis With Spectrophotometric Detection"
Bull. Chem. Soc. Jpn. 1993 Volume 66, Issue 3 Pages 966-968
Edison Munaf, Wenzhi Hu and Hiroki Haraguchi

Abstract: Sample (8 to 40 g) was digested using 40 mL of 1 M HClO4, shaken vigorously and centrifuged at 3000 rpm for 5 min. A 20 mL portion of the supernatant solution was adjusted to pH 6.5 with 1.5 M potassium hydrogen carbonate and a 20 µL portion of this solution was injected into a carrier stream which merged with a stream (100 µL min-1) of 4% potassium peroxodisulfate solution and passed through a PTFE reaction coil (8 m x 0.5 mm) at 140°C. The mixture then merged with a stream (100 µL min-1) of 2% ammonium molybdate solution containing 0.36% ascorbic acid and 1.5 M H2SO4 and passed through a second PTFE coil (5 m x 0.5 mm) before the absorbance was measured at 880 nm. The calibration graph was rectilinear for 0.5 to 20 µg mL-1 of P; the detection limit was 16 ng mL-1. The method was used to determine P in chicken heart, eggs of salmon and yellowtail fish and in livers of cow, pig, chicken and fish.
Phosphorus Sample preparation Spectrophotometry PPB Heated reaction