University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> kidney -> rat

Citations 2

"Quantitative Determination Of Unchanged Cisplatin In Rat Kidney And Liver By High Performance Liquid Chromatography"
J. Chromatogr. B 1995 Volume 663, Issue 1 Pages 181-186
Kazuhiko Hanada, Naomi Nagai and Hiroyasu Ogata*

Abstract: A quantitative analytical method for measuring unchanged cisplatin (CDDP) and high- and low-molecular-mass metabolites (fixed and mobile metabolites) in rat kidney and liver was developed. Unchanged CDDP, separated from fixed and mobile metabolites in tissue homogenates by consecutive procedures of fractionation and ultrafiltration, was determined by high performance liquid chromatography (HPLC) with post-column derivatization. Although unchanged CDDP was found to be partly metabolized to fixed metabolites during the preparation of cytosolic ultrafiltrates, the recovery of unchanged CDDP gave a constant value (about 70%), which was independent of tissue type and CDDP concentration (from 1 to 10 µg/ml). The detection limit for unchanged CDDP in the cytosolic ultrafiltrate was 20 ng/ml, corresponding to a concentration detection limit of 65 ng Pt per g of tissue in the kidney and liver. The concentrations of fixed and mobile metabolites were determined as platinum concentrations in the tissue homogenate and in the cytosolic ultrafiltrate using atomic absorption spectrometry after correcting for transformation of unchanged CDDP to fixed metabolites. The distribution of unchanged CDDP, mobile metabolites and fixed metabolites in rat kidney and liver, after bolus injection of CDDP (5 mg/kg), was determined using this method.
Cis-platin Cisplatin, metabolites HPLC Post-column derivatization

"Separation Of Ascorbic Acid, Isoascorbic Acid, Dehydroascorbic Acid And Dehydroisoascorbic Acid In Food And Animal Tissue"
J. Micronutr. Anal. 1990 Volume 7, Issue 1 Pages 67-70
Vanderslice, J.T.;Higgs, D.J.

Abstract: Samples (fresh broccoli and rat liver and kidney) were extracted with 8% acetic acid - 3% HPO3, the extracts were washed with hexane, with centrifugation, and the acid layers were analyzed on two PLRP-S (5 µm) columns (15 cm x 4.6 mm and 25 cm x 4.6 mm) in series operated at 4°C with 0.2 M NaH2PO4 (pH 2.14) as mobile phase (0.5 mL min-1). Post-column derivatization was carried out by oxidation with HgCl2 and subsequent reaction with o-phenylenediamine; detection was by fluorescence at 430 nm (excitation at 350 nm). Baseline separation of the cited analytes was achieved for both standards and sample extracts.
Ascorbic acid isoascorbic acid dehydroascorbic acid dehydroisoascorbic acid Fluorescence Column Post-column derivatization