University of North Florida
Browse the Citations

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

View Stuart Chalk's profile on LinkedIn


Classification: Biological tissue -> hepatopancreas -> lobster

Citations 2

"Determination Of Total Mercury In Biological Tissues By Flow Injection Cold Vapor Generation Atomic Absorption Spectrometry Following Tetramethylammonium Hydroxide Digestion"
Analyst 1998 Volume 123, Issue 6 Pages 1215-1218
Guanhong Tao, Scott N. Willie and Ralph E. Sturgeon

Abstract: A simple, rapid and reliable method was developed for the determination of total mercury in biological samples. Samples were solubilized by tetramethylammonium hydroxide (TMAH). The organically bound mercury was cleaved and converted to inorganic mercury by online addition of KMnO4. The decomposed mercury together with inorganic mercury originally present in samples was determined by flow injection cold vapor atomic absorption spectrometry after reduction to elemental mercury vapor using NaBH4. A sample throughput of 100 measurements per h was achieved after a 30 min dissolution with TMAH. The relative standard deviation for 20 µg L-1 Hg was 1.3% and the limit of detection was 0.1 µg L-1 (3s). The proposed method was validated by the anal. of a suite of certified marine biological reference materials, DORM-2 (dogfish muscle), DOLT-2 (dogfish liver) and TORT-2 (lobster hepatopancreas), with calibration against simple Hg(II) standards.
Mercury Sample preparation Spectrophotometry Reference material Volatile generation

"Continuous-flow Microwave-assisted Digestion Of Environmental Samples Using Atomic Spectrometric Detection"
J. Anal. At. Spectrom. 1995 Volume 10, Issue 11 Pages 981-986
Ralph E. Sturgeon, Scott N. Willie, Brad A. Methven, Joseph W. H. Lam and Henryk Matusiewicz

Abstract: A continuous-flow microwave-assisted digestion technique was tested with a view to the evaluation of its effectiveness for decomposition of environmental samples. A CEM SpectroPrep system was used at moderate powers and pressures of up to 2413 kPa to perform on-line digestion of slurried samples of biological tissues (0.5% m/v) and marine sediment (1% m/v). The efficiency of oxidation of biological matrices, as characterized by the residual carbon content of the solutions, was 64%. Recovery of trace elements averaged 90±1% and was accommodated with the use of suitable internal standards. Accuracy was verified by analysis of certified reference materials from the National Research Council of Canada, marine sediment BCSS-1 and lobster hepatopancreas tissue LUTS-1. Precision of measurement, as reflected in the determination of the trace metal content in replicate solutions, using a variety of atomic spectrometric techniques, was better than 1% RSD (relative standard deviation).
Metals Sample preparation Spectrophotometry Microwave Online digestion