University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Fish Tissue

Classification: Biological tissue -> fish -> freeze dried

Citations 1

"Determination Of Arsenic And Selenium In Whole Fish By Continuous-flow Hydride-generation Atomic Absorption Spectrophotometry"
J. AOAC Int. 1989 Volume 72, Issue 3 Pages 484-486
Brumbaugh WG, Walther MJ

Abstract: Freeze-dried homogenized fish tissue (~0.5 g) and 2 to 3 mL of methanol were mixed with 5 drops of anti-foaming agent, 10 mL of 40% Mg(NO3)2.6H2O and 10 mL of HNO3, and the mixture was heated under reflux at 70°C to 80°C overnight. The temperature was increased to 200°C, the mixture was evaporated to dryness and the residue was placed in a cold muffle furnace. The temperature was increased to 500°C over 3 to 4 h (held for 2 to 4 h), the mixture was cooled, 20 mL of 50% HCl was added and the solution was boiled for 1 h on a hot plate. After cooling, the volume was adjusted to 20 mL with 50% HCl and As and Se in the solution were determined by hydride-generation AAS at 193.7 and 196.0 nm, respectively, with 0.6% NaBH4 - 0.5% NaOH and 1% NaBH4 - 0.5% NaOH as reductants for Se and As, respectively. Detection limits were ~0.06 and ~0.04 µg g-1 for As and Se, respectively, and the average coefficient of variation for both elements was 4%.
Arsenic Selenium Sample preparation Spectrophotometry Volatile generation Volatile generation