University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> brain -> mouse

Citations 2

"Application Of Automated Flow Injection Analysis To Determine Nitrite And Nitrate In Mouse Brain"
Neuroreport 1994 Volume 5, Issue 13 Pages 1571-1573
Habu H, Yokoi I, Kabuto H, Mori A.

Abstract: Nitric oxide level in the mouse brain was estimated by determination of nitrite and nitrate using an automated flow injection analyzer. for NOx. Different experimental conditions were examined to determine which produced reproducible results. After pretreatment of tissue specimens by the ZnSO4-NaOH method for deproteinization, reproducible and constant values were obtained. The values were more accurate immediately after sectioning without freezing than after 24 h with freezing. Two sacrifice methods, decapitation and microwave irradiation of the head, were investigated, but there was no significant difference between the two. No substances in the mouse brain exerted a positive or negative influence on the results. These results show that our method is indeed applicable to the brain tissue.
Nitrate Nitrite

"Determination Of O-phosphoethanolamine In Male And Female Mouse Tissues By High Performance Liquid Chromatography"
J. Pharm. Soc. Jpn. 1986 Volume 106, Issue 8 Pages 694-697

Abstract: Phosphoethanolamine was extracted from mouse tissues (e.g., brain, salivary gland, thymus, liver, spleen and kidney) with trichloroacetic acid before determination by cation-exchange chromatography on a column (15 cm x 4 mm) of Shimadzu ISC-07/S at 55°C with, as mobile phase, 0.2 M Na citrate - HClO4 buffer (pH 2.0) containing 7% of ethanol and 0.01% of octanoic acid at 0.3 mL min-1. Fluorimetric detection was used with post-column derivatization with phthalaldehyde. Recovery was 93.8 to 103.8%.
o-Phosphoethanolamine HPIC Fluorescence Heated reaction Post-column derivatization