University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological material -> humor -> human

Citations 1

"Determination Of Iron, Copper, And Zinc In Human Whole Vitreous Humor By Flow Injection Atomic Absorption Spectrometry"
Acta Cient. Venez. 1988 Volume 39, Issue 3 Pages 224-229
Burguera JL, Burguera M, Rivas C, Alarcon OM, Ibarra de Diaz B

Abstract: Vitreous humor is analized for iron, copper and zinc by flow injection atomic absorption spectrometry. Optimal conditions for the determination of these elements were established. The sample is injected as a discrete slug into a carrier stream of water. Standards and samples are matched in physiological concentrations of some chemical species. The effect of viscosity was studied by preparating standards in glycerol, hyaluronic acid and chondroitin sulfate at different concentrations. Glycerol was found to be more convenient for preparation of viscosity-controlled standards. Recoveries are 95 to 105%. The flow injection determination of iron, copper and zinc in vitreous body is reproductible, simple and quick, can be performed using microsamples and give more precise results than conventional colorimetric methods.
Copper Iron Zinc Clinical analysis Spectrophotometry