University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Hippocampus Perfusate

Classification: Biological material -> hippocampus -> perfusate

Citations 2

"Flow-through Fiber-optic Ammonia Sensor For Analysis Of Hippocampus Slice Perfusates"
Anal. Chim. Acta 1997 Volume 357, Issue 1-2 Pages 79-84
Scott K. Spear, Shawnna L. Patterson and Mark A. Arnold*

Abstract: An absorbance based flow-through fiber-optic probe is developed for measurements of ammonia in neurochemical samples. The probe is constructed by trapping a small volume of an internal indicator solution between two sets of optical fibers. This indicator solution is separated from a flowing sample stream by a microporous gas permeable membrane. The flow-through design permits analysis of small aliquots of sample which are injected and pass across the gas permeable membrane. Ammonia in the sample diffuses across this membrane, enters the internal solution, and alters the distribution of a pH sensitive chromophore. The resulting change in absorbance is measured and related to the sample ammonia concentration. The final ammonia sensor possesses a 0.2-20 µM dynamic range with a detection limit of 0.2 ± 0.1 µM and response time of 8 min. The utility of this ammonia sensor is illustrated by measuring extracellular ammonia levels from perfused rat hippocampal tissue. These measurements indicate extracellular ammonia levels fluctuate during tissue depolarization, which is consistent with our findings with other neurological tissues.
Ammonia Electrode Sensor Optical fiber

"Quantitative On-line Monitoring Of Hippocampus Glucose And Lactate Metabolism In Organotypic Cultures Using Biosensor Technology"
J. Neurochem. 2003 Volume 85, Issue 2 Pages 399-408
Jan Bert Gramsbergen,* Gea Leegsma-Vogt, Kor Venema, Jens Noraberg and Jakob Korf

Abstract: Quantitative glucose and lactate metabolism was assessed in continuously perfused organotypic hippocampal slices under control conditions and during exposure to glutamate and drugs that interfere with aerobic and anaerobic metabolism. On-line detection was possible with a system based on slow perfusion rates, a half-open (medium/air interface) tissue chamber and a flow injection analytic system equipped with biosensors for glucose and lactate. Under basal conditions about 50% of consumed glucose was converted to lactate in hippocampal slice cultures. Using medium containing lactate (5 mM) instead of glucose (5 mM) significant lactate uptake was observed, but this uptake was less than the net uptake of lactate equivalents in glucose-containing medium. Glucose deprivation experiments suggested lactate efflux from glycogen stores. The effects of drugs compromising or stimulating energy metabolism, i.e. 2-deoxyglucose, 3-nitropropionic acid, α-cyano-4-hydroxycinnamate, l-glutamate, d-asparate, ouabain and monensin, were tested in this flow system. The data show that maintaining Na+ and K+ gradients consumed much of the energy but do not support the hypothesis that l-glutamate stimulates glycolysis in hippocampal slice cultures.
Glucose Lactate Sensor Interferences