University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Gingival Human

Classification: Biological material -> fibroblast -> gingival -> human

Citations 1

"Improved High Performance Liquid Chromatography Method For Quantitation Of Proline And Hydroxyproline In Biological Materials"
J. Chromatogr. A 1986 Volume 356, Issue 1 Pages 163-170
K. Yaegaki, J. Tonzetich and A. S. K. Ng

Abstract: Test samples were derivatized with phthalaldehyde(I) to remove primary amino-acids and subjected to HPLC on a column (10 cm x 8 mm) of Nova-Pak C18 Radial-Pak with gradient elution with acetonitrile containing acetate buffer solution (pH 6.4) at 1.5 mL min-1. The secondary amino-acid-containing fractions were collected, freeze-dried and derivatized with phenyl isothiocyanate. The resulting mixture was evaporated to dryness, and the residue was dissolved in phosphate buffer and analyzed on the same column as before. Detection was at 254 nm. Proline(I) and hydroxyproline(II) were well resolved with good peaks; recovery was 93% provided that the analysis was initiated within 5 min of I derivatization. The method was evaluated by using human gingival fibroblast cultures and was found to be particularly useful for the analysis of low concentration. of I and II in the presence of large amounts of primary amino-acids. Compared with other methods, this technique has higher sensitivity and recovery, no interference and no need for post-column derivatization.
Hydroxyproline l-Proline HPLC Spectrophotometry Interferences