University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Faeces

Classification: Biological material -> faeces

Citations 1

"High Performance Liquid Chromatographic Determination Of Clenbuterol And Cimaterol Using Post-column Derivatization"
J. Chromatogr. B 1991 Volume 564, Issue 2 Pages 537-549
Dirk Courtheyn*, Carlo Desaever and Roland Verhe

Abstract: Animal tissues, faeces and feeding-stuffs were extracted with dilute 0.5 M HCl saturated with ethyl acetate; the resulting extracts or liquid samples, e.g., urine, plasma, blood and bile were purified on Chem Elut CE 120 columns and eluted with toluene - CH2Cl2. The eluate was mixed with 0.1 M HCl, ultrasonicated and centrifuged before a portion of the mixture was analyzed by HPLC on a column (15 cm x 4.6 mm) of Nova-Pak C18 (4 µm) with 25 mM sodium dodecyl sulfate and 0.02 M anhydrous acetic acid buffer of pH 3.5 containing 1 M NaOH - acetonitrile (53:47) as mobile phase (1.3 mL min-1). The eluate was derivatized with use of three reagents (details given) before the absorbance was measured at 537 and 493 nm for cimaterol and clenbuterol, respectively. Detection limits for liquid and solid samples were 0.1 ng mL-1 and 0.2 ng g-1, respectively. Results agreed well with those obtained by high performance TLC and GC - MS.
Cimaterol Clenbuterol HPLC Spectrophotometry Buffer Column Detection limit pH Post-column derivatization