University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Cell

Classification: Biological material -> cell

Citations 1

"Configurations Of A Flow Injection System For Perfusion Studies Of Adherent Cells"
Anal. Chem. 1995 Volume 67, Issue 8 Pages 1486-1490
Pamela J. Baxter, Lars Hallgren, Cy H. Pollema, Michael Trnka, and Jaromir Ruzicka

Abstract: Three instrument configurations are described for flow injection apparatus designed to be the fluidic drive for a flow-through perfusion chamber for live cell perfusion with fluorescence microscopy detection. The instrument consists of two linear syringe pumps and a six-port selection valve which, under computer control, allowed sequential injection, fluid switching or flow injection to be performed with minimal system reconfiguration. With a dual-inlet perfusion chamber, target cells could be exposed to very steep reagent pulses; single-inlet chambers allowed more flexibility and a gradual increase in reagent concentration. This paper describes three configurations of a flow injection apparatus designed to be the fluidic drive for perfusion studies of cultured adherent cells. The apparatus was coupled to a flow-through perfusion chamber that was specifically designed for live cell perfusion using fluorescence microscopy as the detector. The instrument consists of two linear syringe pumps and a multiposition selector valve which, under computer control, allowed sequential injection, fluid switching, and flow injection to be performed with minimal system reconfiguration. When the apparatus is coupled to a dual inlet perfusion chamber, target cells can be exposed to very steep reagent pulses, while the traditional single inlet perfusion chamber allows more flexibility and provides a more gradual increase in reagent concentration. The most significant salient feature of the system is the ability to generate very steep pulses--a desirable feature for cell perfusion studies.
Cells Flowcell Sequential injection Pulsed reagent Perfusion