University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological fluid -> urine -> human -> baby

Citations 1

"Routine Assay Of Creatinine In Newborn Baby Urine By Spectrophotometric Flow Injection Analysis"
Anal. Chim. Acta 1995 Volume 308, Issue 1-3 Pages 446-450
Tadao Sakai*, Hiroshi Ohta, Noriko Ohno and Junzo Imai

Abstract: An FIA method based on the Jaffe reaction was described for the determination of creatinine in urine. Urine was collected on a soft adsorbent cotton pad. The soaked pad was wrung to recover 0.5 mL urine. The urine sample was diluted with 4.5 mL 0.03 M KH2PO4 containing 0.4% EDTA (carrier solution). A 100 µL portion of this solution was injected into the carrier stream (0.82 ml/min) which was merged with the reagent stream (0.82 ml/min) containing 0.01 M sodium picrate in 1.5% NaOH. The flow passed through the reaction coil (1.5 m x 0.5 mm i.d.) at 50°C to the detector where the absorbance was measured at 515 nm using a 10 mm pathlength flow cell. The calibration graph was linear for 5-200 µg/ml of creatinine with a detection limit of 3 µg/ml. The RSD (n = 10) for the determination of 50 µg/ml creatinine was 0.6%. The results were consistent with those obtained by a batchwise experiment and LC.
Creatinine Spectrophotometry Heated reaction Method comparison