University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Subcutaneous Fluid

Classification: Biological fluid -> subcutaneous

Citations 1

"A Microdialysis Probe Coupled With A Miniaturized Thermal Glucose Sensor For In Vivo Monitoring"
Anal. Lett. 1995 Volume 28, Issue 13 Pages 2275-2286
Amine, A.;Digua, K.;Xie, B.;Danielsson, B.

Abstract: A miniaturized thermal biosensor was coupled with a microdialysis probe (MP) for subcutaneous glucose (I) monitoring. The thermal biosensor measured the heat evolved during enzyme catalyzed reactions and consisted of a stainless steel column (15 mm x 1.5 mm i.d.) filled with controlled pore glass beads (125-140 µm) onto which glucose oxidase and catalase had been co-immobilized. The temperature signal was measured with a Wheatstone bridge. The biosensor was incorporated into a FIA system equipped with a 1 µL sample loop and operated with 0.1 M sodium phosphate buffer carrier stream (60 µL/min). The MP was fitted with a polycarbonate-polyether co-polymeric membrane (1.6 cm x 0.5 mm i.d., 20 000 Da molecular weight cut-off) and 0.1 M PBS at pH 7.2 was used as the perfusion fluid at 3 µL/min. The thermal sensor exhibited a linear response from 1-50 mM I and a response time of ~e;85 s. In vivo experiments were performed by inserting a 1 cm portion of the MP hollow fiber into the skin of a healthy volunteer who had been orally dosed with I and the levels of I in subcutaneous fluid was measured at 5 min intervals over a period of 180 min after dosage and the results were compared with levels of I in blood measured at 20 min intervals. Subcutaneous concentrations were found to follow the blood concentrations with a time lag of 18 min.
Glucose Sensor Thermistor Dialysis Controlled pore glass Membrane In vivo monitoring Perfusion