University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Haemolymph Fluid

Classification: Biological fluid -> haemolymph

Citations 1

"A Technique For The Measurement Of Total Ammonia In Small Volumes Of Seawater And Haemolymph"
Ophelia 1993 Volume 37, Issue 1 Pages 31-40
Hunter, D.A.;Uglow, R.F.

Abstract: A rapid and precise technique combining flow injection analysis and gas diffusion is described for the measurement of total ammonia (NH-3 + NH-4+) in small volumes (100-450 µl) of seawater and diluted haemolymph. Calibration of the method with seawater ammonia standard solution in range 5.0-50.0 µmol ammonia L-1 (n = 6) gave linear calibration curves (r = 0.999 in each case) with precision in the range 0.2-3.0%. Precision of measurement of ammonia in samples of seawater and diluted haemolymph was 0.9-3.3% (n = 11-13). The lower limit of detection is 0.20 µmol ammonia l-1. Thirty determinations can be made per hour.
Ammonia, total Gas diffusion Small sample