University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Gingival Fluid

Classification: Biological fluid -> gingival

Citations 1

"Liquid Chromatographic And Flow Injection Analysis Of Tetracycline Using Sensitized Europium(III) Luminescence Detection"
J. Chromatogr. B 1988 Volume 433, Issue 1 Pages 149-158
Thomas J. Wenzel, Lisa M. Collette, Deirdre T. Dahlen, Susan M. Hendrickson and Lawrence W. Yarmaloff

Abstract: Europium was used as a fluorophore for the detection of tetracycline(I) in HPLC and flow injection analysis. Gingival fluid was absorbed on filter-paper strips and tetracycline(I) was extracted with 1 mM Na2EDTA in 0.12 M KNO3 - acetonitrile (9:1). HPLC was performed on a column (25 cm x 4.6 mm) of C8 silica (10 µm). Various mobile phases and post-column reagent solution containing Eu(III) were evaluated; the fluorescence was detected at 616 nm (excitation at 392 nm). A 30 µs delay was incorporated in fluorescence measurement to avoid interference from fluorescent impurities. Detection limits were better than those obtained with UV detection. Europium (III) can be used as a luminescent chromophore for detection in the liquid chromatographic and flow injection analysis of tetracycline. Detection is dependent upon an intramolecular energy transfer from the tetracycline to Eu (III). In liquid chromatography, the Eu (III) is added post-column as a complex with ethylenediaminetetraacetic acid. The post-column phase also serves to adjust the pH for optimum sensitivity. The method is highly selective for tetracycline since few compounds are capable of transferring energy to Eu (III). Fluorescent impurities that would otherwise interfere in flow injection analysis can be eliminated through the use of a delay time between the source pulse and the start of data acquisition. The detection limits for tetracycline using sensitized Eu (III) luminescence are better than those obtained using ultraviolet detection. The method is applied to the analysis of tetracycline in urine, blood serum, and gingival crevice fluid.
Tetracycline HPLC Fluorescence Luminescence Complexation Interferences Post-column derivatization