University of North Florida
Browse the Citations

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

View Stuart Chalk's profile on LinkedIn

Cerebrospinal Fluid

Classification: Biological fluid -> cerebrospinal -> rat

Citations 1

"An Enzyme-reactor For Electrochemical Monitoring Of Choline And Acetylcholine: Applications In High Performance Liquid Chromatography, Brain Tissue, Microdialysis And Cerebrospinal Fluid"
Anal. Biochem. 1992 Volume 204, Issue 2 Pages 305-310
Frans Flentge, Kor Venema, Tineke Koch and Jakob Korf

Abstract: A sandwich-type enzyme reactor in which the enzymes are physically immobilized in a minimal dead space between two cellulose membranes, resulting in improved sensitivity, was developed for the electro-chemical detection of choline (Ch) and acetylcholine (ACh). The reactor contains the enzymes choline oxidase with or without acetylcholine esterase, for the detection of ACh and Ch, respectively. For the HPLC analysis of Ch and ACh the detection system was coupled post-column. Levels of Ch and ACh of rat striatum tissue and human cerebrospinal fluid were found to be similar to those determined with published methods. Because of low back pressure--a further advantage of the reactor--the detection system could also be directly coupled to the outlet of a microdialysis device, allowing the online real-time measurement of extracellular brain Ch. The versatility of the enzyme reactor for the monitoring of analytes in HPLC eluates, flow injection analysis, with or without prepurification, is emphasized. The usefulness of the reactor-detector system in biomedical applications is illustrated by the measurement of increases of rat striatal extracellular Ch following cardiac arrest.
Choline Acetylcholine HPLC Electrochemical analysis Post-column derivatization Immobilized enzyme Dialysis