University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Rat Fluid

Citations 1

"Measurement Of Histamine In Rat Bronchoalveolar Lavage Fluid By High Performance Cation-exchange Chromatography Coupled With Post-column Derivatization To O-phthaldialdehyde"
J. Chromatogr. B 1992 Volume 574, Issue 2 Pages 340-343
T. A. Neubecker, N. S. Miller, T. N. Asquith* and K. E. Driscoll

Abstract: Histamine (I) was determined in bronchoalveolar lavage fluid (100 µL) by cation-exchange HPLC on a column (10 cm x 4.6 mm) of Partisil 5 SCX RAC II (5 µm) with Si-saturated 0.17 M KH2PO4 as mobile phase (1 mL min-1). The column eluate was mixed with phthalaldehyde reagent solution (pH 11.7 to 12.0) in a turbulent mixer and the reaction mixture was passed to a reaction coil (305 cm x 0.5 mm) maintained at 65°C. Fluorimetric detection was at 418 nm (excitation at 230 nm). The calibration graph was rectilinear from 1 to 65 ng mL-1 of I. The coefficient of variation (n = 4) were 1.8 to 6.0% for 3.28 to 16.4 ng mL-1 of I. Day-to-day coefficient of variation were 2.7 to 6.4%. Average recovery was 94.2%.
Histamine HPLC Fluorescence Post-column derivatization Heated reaction