University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Serum Rat

Classification: Biological fluid -> blood -> serum -> rat

Citations 2

"Fluorimetric Determination Of 3,5-dimethyl-4,6-diphenyltetrahydro-2H-1,3,5-thiadiazine-2-thione In Rat Serum By High Performance Liquid Chromatography With Post-column Derivatization"
Bunseki Kagaku 1986 Volume 35, Issue 4 Pages 384-388
Nakajima, A.;Ushijima, T.;Migita, J.

Abstract: Rat serum was extracted with CHCl3, and the organic phase was evaporated to dryness. The residue was dissolved in methanol and subjected to HPLC, at 50°C, on a column (15 cm x 4.6 mm) of Zorbax ODS (5 µm) with a mobile phase of aqueous 80% methanol at 0.7 mL min-1. After detection at 285 nm, the eluate was heated at 50°C with 0.1% of NaClO in 0.15 M borate buffer (pH 10.5) and the solution was mixed with phthalaldehyde - 2-mercaptoethanol reagent; detection was by measurement of the fluorescence at 450 nm (excitation at 335 nm). The calibration graph was rectilinear for 0.2 to 10 ng of tetrahydro-3,5-dimethyl-4,6-diphenyl-2H-1,3,5-thiadiazine-2-thione, and the limit of determination was 2.5 ng mL-1. The coefficient of variation was 5.02 to 6.97%.
3,5-dimethyl-4-6-diphenyltetrahydro-2H-1-3-5-thiadiazine-2-thione HPLC Fluorescence Spectrophotometry Heated reaction Post-column derivatization

"Simultaneous Determination Of 2-deoxyglucoseSimultaneous Determination Of 2-deoxyglucose"
Chem. Pharm. Bull. 1990 Volume 38, Issue 4 Pages 963-965
Umegae, Y.;Nohta, H.;Ohkura, Y.

Abstract: Sample solution (0.1 ml) was injected on to a TSK gel Sugar AXG column (15 cm x 4.6 mm) operated at 60°C with 1 M borate buffer (pH 9.0) as mobile phase (0.4 mL min-1). The effluent was mixed with 15 mM meso-1,2-bis-(4-methoxyphenyl)ethylenediamine in aqueous 30% ethanol - 0.6 M NaOH (0.3 mL min-1) and passed through a reaction coil (20 m x 0.5 mm) at 140°C and a cooling coil (1 m x 0.5 mm), and the fluorescence was measured at 460 nm (excitation at 330 nm). Calibration graphs were rectilinear for up to 100 nmol injected, the detection limits for 2-deoxy-D-ribose, 2-deoxyglucose (I), rhamnose, L-fucose and glucose (II) were 21, 26, 27, 18 and 28 pmol, respectively, and the respective coefficient of variation (n = 10) at 1 nmol were 1.7, 1.5, 2.1, 1.5 and 1.9%. The method was applied to determine I and II in deproteinized serum, with L-fucose as internal standard.
Glucose 2-Deoxyglucose HPLC Fluorescence Post-column derivatization Column Calibration Detection limit Internal standard