University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Serum Pig

Classification: Biological fluid -> blood -> serum -> pig

Citations 3

"Quartz Crystal Biosensor For Detection Of The African Swine Fever Disease"
Anal. Chim. Acta 1998 Volume 362, Issue 1 Pages 91-100
Erich Uttenthaler*, Conrad Kößlinger and Stephan Drost

Abstract: An immunosensor for the detection of the African Swine Fever (ASF) disease in infected pigs is presented. A sensitive, direct immunoassay for measurements in diluted pig sera was established using the virus protein 73 (VP73) as a highly specific receptor layer for a mass sensitive piezoelec. quartz crystal. The fundamental sensor effect of this transducer is based on the linear dependence of the resonance frequency of an oscillating quartz crystal upon the binding of mass on the coated surface during the measurement. A quartz crystal which is coated with VP73 is an highly specific sensor and allows to detect the ASF-antibodies in the sample through mass accumulation on the surface of the quartz crystal. An appropriate immobilization technique for VP73 was established and a suitable carrier buffer for the flow injection analysis system was developed during this study. Regeneration of the receptor layer is possible for about ten times. With this quartz crystal microbalance, results were available within a few minutes and with a selectivity comparable to a licensed microtiterplate ELISA. Measurements with real pig serum samples have proven the suitability of the quartz crystal biosensor for the classification ofpositive and negative pig serum samples.
African swine fever virus Protein Microbalance Sensor Immunoassay Buffer Optimization Method comparison

"Electrochemical Detection Of African Swine Fever Virus In Pig Serum With A Competitive Separation Flow Injection Analysis Immunoassay"
Analyst 1997 Volume 122, Issue 2 Pages 155-159
Matthias Stiene and Ursula Bilitewski

Abstract: Pig serum was diluted 50-fold with PBS containing 0.05% Tween 20. A portion (1 ml) of the resulting solution was incubated with 50 ng biotinylated virus protein VP73 (preparation described) and 250 ng horse-radish peroxidase-labelled mAb 18BG3 (Ingenasa) for 25 min. A portion of the reaction mixture was injected into a carrier stream of 0.1 M phosphate buffer of pH 5.5 (buffer A) and passed through a column of biotinylated glass beads coated with streptavidin (preparation described) where the immunological complex formed during the incubation reaction was trapped. The peroxidase activity of the captured labelled antibodies was determined by passing a solution of H2O2 and hydroquinone (each 2 mM) in buffer A through the column and detecting the enzymatic product amperometrically at a vitreous C electrode at -100 mV vs. Ag/AgCl. The calibration graph was linear from 1-80 ng/ml mAb 18BG3 with a concentration of 50% inhibition at 6 ng/ml.
African swine fever virus Immunoassay Amperometry Electrode Column Glass beads Buffer

"Electrochemical Detection Of Pyrroloquinoline Quinone Coupled With Its Catalytic Function By Liquid Chromatography"
Anal. Sci. 1993 Volume 9, Issue 2 Pages 207-211

Abstract: Samples of milk and swine serum were deproteinized by addition of 30% trichloroacetic acid. After centrifuging, the supernatant solution was acidified with HCl to pH 0.5 and extracted with butanol. A portion (20 µL) was analyzed by HPLC on a Cosmosil 5C-18AR column (25 cm x 4.6 mm) with methanol - 6 mM H3PO4 (3:7) containing 5 µM-K3Fe(CN)6 as mobile phase (1 mL min-1). Post-column derivatization was carried out by mixing the column eluate with a stream (0.2 mL min-1) of 3 M glycine - 2.5 M ammonium buffer. The reaction coil consisted of a Teflon tube (17.5 m x 0.5 mm) maintained at 25°C. Electrochemical detection was used with a vitreous-carbon working electrode, a Ag - AgCl reference electrode and a stainless-steel tube counter electrode. Calibration graphs were rectilinear from 0.2 pM- (detection limit) to 6 µM-pyrroloquinoline quinone. The method was also applied to table vinegar.
Pyrroloquinoline-quinone HPLC Electrode Catalysis Post-column derivatization