University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Serum Horse

Classification: Biological fluid -> blood -> serum -> horse

Citations 1

"Simultaneous Spectrophotometric Determination Of Iron And Copper In Serum With 2-(5-bromo-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)aniline By Flow Injection Analysis"
Anal. Chim. Acta 1992 Volume 261, Issue 1-2 Pages 197-203
Sam Woo Kang, Tadao Saki* and Noriko Ohno, Kazunori Ida

Abstract: The system described and illustrated incorporates a double-beam spectrophotometer with two flow cells. The sample is injected into 0.1 M HCl - 0.1 mM KIO4 as carrier and mixed with a 0.1 mM solution of the cited reagent in acetate buffer of pH 4.5 in a 15-cm reaction coil. The solution is passed through the first flow cell for measurement of the absorbance due to Cu(II) at 558 nm. A 10 mM solution of Na ascorbate in the buffer is introduced into the stream, which passes through a 700-cm reaction coil heated at 60°C before measurement of the absorbance at 558 nm in the second flow cell; in this instance an inverted peak for Fe(II) and a positive peak for Cu(II) are obtained. The Fe(III) present after adding the sample to the carrier does not interfere. The calibration graphs are rectilinear for 50 to 200 µg L-1 of Cu(II) or Fe(II) and the limit of detection is 2.4 µg l-1. Nickel and Co interfere at concentration. >10 µg L-1 which, however, are not encountered in serum samples. Serum is deproteinized with trichloroacetic acid at 90°C before analysis. Results on human, horse, bovine, chicken and goat serum agreed with those obtained by ICP-AES. A method for the simultaneous spectrophotometric determination of iron and copper by flow injection analysis was developed using 2-(5-bromo-2-pyridylazo)-5-(N-propyl-N- sulfopropylamino)aniline. The flow system utilizes a double-beam spectrophotometric detector with two flow cells. The reagent forms water-sol. chelates with Cu(II) and Fe(II) in an acetate-buffered medium at pH 4.5. The molar absorptivities of the complexes are 65,000 L mol-1 cm-1 at 578 nm for Cu(II) and 87,000 L mol-1 cm-1 at 558 nm for Fe(II). In one reaction coil Fe(II) is oxidized to Fe(III) by KIO4, so the Fe(II) chelate is not formed and only the colored complexes with Cu(II) are monitored in the first flow cell. In a second reaction coil, the solution merges with sodium ascorbate solution to reduce Cu(II) and Fe(III) to Cu(I) and Fe(II). Thus only the Fe(II) complex is measured in the second flow cell. Cu(II) and Fe(II) in the range 50-200 µg L-1 are determined at 558 nm. The sample throughput is 30 h-1 with a precision of 1.2%.
Iron Copper Spectrophotometry Simultaneous analysis Method comparison Buffer Complexation Interferences