University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Dog Serum

Classification: Biological fluid -> blood -> serum -> dog

Citations 1

"Determination Of Copper(II) In Serum With 4,4',4',4'''-(21H,23H-porphine-5,10,15,20-tetrayl) Tetrabenzenesulfonic Acid By FIA With Spectrophotometric Detection"
Bunseki Kagaku 1995 Volume 44, Issue 10 Pages 797-801
Adachi, H.;Igarashi, S.;Yotsuyanagi, T.

Abstract: Serum (0.2 ml) is mixed with 0.1 mL each of 1 M HCl and, after 5 min, 1.2 M TCA, and centrifuged at 2000 rpm for 5 min. Supernatant solution (0.1 ml) is mixed with 0.1 mL of 1 M ammonium acetate buffer, and 50 µL of the mixture is injected into a carrier stream (1 ml/min) of a 10 µM solution of the cited reagent in 0.1 M ammonium acetate buffer of pH 6.0 containing also 10 mM ascorbic acid. The stream passes through a 50 cm mixing coil and then merges with a stream (0.5 ml/min) of 1 M TCA, and the mixed stream passes through a 45 cm mixing coil to an 8 µL flow-cell (optical pathlength 1 cm) for detection at 413 nm. The RSD (n = 5) was 1.6%. The result for Cu(II) in deproteinized dog serum agreed with that obtained by flame AAS, and for Cu(II) in deproteinized NIES No.4 freeze-dried serum agreed with that obtained by flame AAS and with the certified value (obtained without deproteinization).
Copper(II) Spectrophotometry Method comparison Reference material