University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Rat Blood

Classification: Biological fluid -> blood -> rat

Citations 3

"Determination Of Thiamine And Its Phosphate Esters In Human And Rat Blood By High Performance Liquid Chromatography With Post-column Derivatization"
J. Chromatogr. A 1985 Volume 332, Issue 1 Pages 181-188
Mieko Kimura and Yoshinori Itokawa

Abstract: Blood, erythrocytes and plasma were deproteinized with aqueous 10% trichloroacetic acid. After centrifugation, the supernatant solution were analyzed by HPLC on a µBondapak C18 column (25 cm x 4 mm) with 0.2 M NaH2PO4 in aqueous 0.3% acetonitrile as the mobile phase (1.0 mL min-1); the eluates were subjected to post-column derivatization with 0.1% of K3Fe(CN)6 in aqueous 15% NaOH and spectrofluorimetric detection at 450 nm (excitation at 375 nm). The detection limit for thiamine and its phosphate esters was 30 fmol and recoveries were 95.0 to 100.2%. The calibration graphs were rectilinear for 50 to 200 fmol.
Thiamine Thiamine triphosphate Thiamine monophosphate HPLC Fluorescence Post-column derivatization

"Improved Flow Injection Analysis Method For Determining Selenium In Biological Samples, And The Effect Of Captopril Administration On Selenium Levels And Glutathione Peroxidase Activity In Rat"
Biol. Pharm. Bull. 1993 Volume 16, Issue 11 Pages 1069-1072
Nobuyuki SUZUKI, Eriko FUJITA, Hiroyuki YASUI, Eiji AOYAMA, Hisashi TANAKA, Terumichi NAKAGAWA

Abstract: An improved flow injection analysis (FIA) method has been developed for the determination of trace selenium in biological samples, and this method has been applied to investigate the effect of captopril, an antihypertensive drug having a thiol group, on selenium concentrations in the rat blood, liver and urine. After oral administration of captopril, selenium levels in the blood decreased, while those in the liver increased significantly. However, no pronounced effect was observed on the urinary excretion rate. The glutathione peroxidase activities in the blood and the liver were comparable to the changes in the selenium levels.
Selenium Captopril

"Methodological Aspects Of Glucose Monitoring With A Slow Continuous Subcutaneous And Intravenous Ultrafiltration System In Rats"
Biosens. Bioelectron. 1997 Volume 12, Issue 9-10 Pages 967-976
Wilhelmina A. Kaptein*, Richard H. A. Kemper, c, Marcel H. J. Ruiters, Kor Venema, Renger G. Tiessen and Jakob Korf

Abstract: A method for the continuous ultrafiltration of venous blood or subcutaneous fluid is demonstrated with glucose monitoring in the living rat. Ultrafiltrate was withdrawn at a constant flow rate of approximately 100 nl/min. Glucose content of the ultrafiltrates was electrochemically determined with a flow injection analysis method and a bi-enzyme reactor. After glucose loading, the time course of glucose in the ultrafiltrate from the jugular vein was virtually identical, whereas that from the subcutaneous compartment was attenuated and the peaks blunted as compared to glucose levels in concomitantly assayed arterial blood. Our study demonstrates the potential of low rate ultrafiltration for monitoring metabolism with biosensor technology in vivo. (C) 1997 Elsevier Science Limited. 21 References
Glucose Sensor Immobilized enzyme Reactor In vivo monitoring Filtration Dialysis