University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Plasma Rabbit

Classification: Biological fluid -> blood -> plasma -> rabbit

Citations 1

"Determination Of Hyaluronic Acid In Rabbit Plasma By HPLC With Fluorimetric Post-column Detection"
Bunseki Kagaku 1989 Volume 38, Issue 2 Pages 92-93
Mitsuma, R.;Yamanashi, S.;Toyoda, H.;Imanari, T.

Abstract: Unsaturated oligosaccharides produced by the enzymatic digestion of hyaluronic acid (I) were separated on a column (15 cm x 4 mm) of TSK gel NH2 60, at 45°C, with 10 mM ammonium formate buffer (pH 5.0) containing 6.5 mM Na2SO4 and 4% of acetonitrile as mobile phase (0.5 mL min-1). After derivatization with 1% 2-cyanoacetamide solution and 0.3 M NaOH at 100°C, fluorescence detection was at 383 nm (excitation at 331 nm). The detection limit was 10 ng of I.
Hyaluronic acid HPLC Fluorescence Sample preparation Post-column derivatization Detection limit