University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Blood Plasma

Classification: Biological fluid -> blood -> plasma -> cow -> calf

Citations 1

"High Performance Liquid Chromatographic Determination Of Spectinomycin In Swine, Calf And Chicken Plasma Using Post-column Derivatization"
J. Chromatogr. B 1995 Volume 672, Issue 1 Pages 165-171
N. Haagsna*, P. Scherpenisse, R. J. Simmonds, S. A. Wood and S. A. Rees

Abstract: An HPLC method for the determination of spectinomycin in swine, calf and chicken plasma at 0.1µg/ml or higher is described. The clean-up is based upon ion-pair solid-phase extraction on a High Hydrophobic C18 column treated with sodium dioctyl sulfosuccinate. After elution with methanol, spectinomycin is chromatographed on a Spherisorb SCX column using 0.1 M sodium sulfate solution (pH 2.6)-acetonitrile (80:20, v/v) as mobile phase. Fluorescence detection is at an excitation wavelength of 340 nm and an emission wavelength of 460 nm after post-column oxidation with sodium hypochlorite followed by derivatization with o-phthaldialdehyde. Mean recoveries were 99±2% (n = 6), 99±2% (n = 7) and 104±2% (n = 6) for swine, calf and chicken plasma, respectively, at the 0.1µg/ml level.
Spectinomycin HPLC Fluorescence Post-column derivatization