University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Human Umbilical Blood

Classification: Biological fluid -> blood -> human -> umbilical

Citations 1

"HPLC Analysis Of Amino-acids With Ion-exchange Chromatography And Phthalaldehyde Post-column Derivatization: Applications To The Assay Of Endogenous Free Amino-acids And Their Transport In Human Placental Villus"
J. Liq. Chromatogr. Relat. Technol. 1986 Volume 9, Issue 8 Pages 1689-1710
B. V. Rama Sastry; V. E. Janson; M. Horst; C. C. Stephan

Abstract: Placental chorionic plate, villus tissue and basal plate homogenates, and umbilical blood samples were deproteinized with acetonitrile. The supernatant solution, after centrifugation, was evaporated under N and the residue was dissolved in sodium diluent (0.27 M Na+; pH 2.2) for analysis on a Waters 840 HPLC Amino Acid System equipped with a column (25 cm x 4.6 mm) of sulfonated polystyrene - divinylbenzene copolymer (equilibrated with Na+). Elution was effected with a gradient of two Na+ buffers of pH 3.15 and 7.4 and post-column derivatization involved phthalaldehyde and 2-mercaptoethanol in borate buffer (pH 10.4). Fluorescence detection was at 410 nm (excitation at 340 nm). A Digital Pro-350 computer made necessary modifications for overlapping or clustered peaks. Analysis took 90 min per sample. Nine essential and six non-essential amino-acids were determined, with rectilinear response from 100 to 500 pmol, and the detection limit was as low as 50 pmol in some instances.
Amino Acids HPLC Fluorescence Post-column derivatization