University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological fluid -> ascites

Citations 1

"Automated Analysis Of Mitomycin C In Body Fluids By High Performance Liquid Chromatography With Online Sample Pretreatment"
J. Chromatogr. A 1987 Volume 420, Issue 1 Pages 53-62
U. R. Tjaden, E. A. De Bruijn, R. A. M. Van Der Hoeven, C. Jol, J. Van Der Greef and H. Lingeman

Abstract: Plasma, ascites fluid and urine were pre-treated by dialysis across a cellulose membrane, followed by concentration. of the mitomycin(I)-containing acceptor stream on pre-columns (3 cm x 2 mm) of Polygosil C8 (40 to 63 µm). Pretreatment was fully automated by using continuous-flow and column-switching techniques. HPLC was carried out on a column (10 cm x 3 mm) of Nucleosil C8 (5 µm) with, as mobile phase, aqueous 15% acetonitrile, and detection at 360 nm. Up to 100 samples a day could be analyzed if two alternating pre-columns were used. The calibration graph was rectilinear from 5 to 200 ng mL-1 and was independent of the biological matrix. Within- and between-day coefficient of variation ranged from 4.4 to 2.9 and 5 to 11.3%, respectively, for plasma. Recoveries were 25% and the limit of determination was 0.5 to 1 ng mL-1. Floxuridine, fluorouracil, doxorubicin, vincristine and bleomycin did not interfere.
Mitomycin C HPLC Sample preparation Spectrophotometry Interferences Membrane Preconcentration Dialysis