University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Amniotic Fluid

Classification: Biological fluid -> amniotic

Citations 2

"Amperometric Flow Injection Determination Of Putrescine And Putrescine Oxidase"
Anal. Chim. Acta 1998 Volume 363, Issue 1 Pages 57-65
Sayed A. M. Marzouk, Clarke X. Xu, Bogdan R. Cosofret, Richard P. Buck*, Saad S. M. Hassan, Michael R. Neuman and Robert H. Sprinkle

Abstract: Flow injection methods for sensitive, accurate and rapid determination of diamines, mainly putrescine (PUT) and cadaverine (CAD), and diamine oxidase (DAO) are described. Putrescine determination is based on an immobilized enzyme reactor (IER) loaded with putrescine oxidase and amperometric detection of the produced hydrogen peroxide at a platinum electrode. This assay was designed for the diagnosis of bacterial vaginosis. Putrescine oxidase (PUO) was assayed by simple injection of putrescine oxidase solution into a carrier buffer containing a fixed concentration. of putrescine followed by similar detection of hydrogen peroxide. In PUT measurements peak currents were linearly related to putrescine concentration. in the range from 10 µM to 1 mM with sample frequency of ~70 samples/h. No change in sensitivity and detection limit were observed after more than 1000 injections over a two week period. PUO was determined in the range of 50-2500 mU/mL, with sample frequency of ~40 samples/h. The lower detection limit of the present method (1.5 mU/mL) is suitable for determining the expected low levels of PUO in the amniotic fluid as a possible marker for premature rupture of membranes (PROM). The effects of flow rates, sample sizes, and substrate concentrations. in the detection limits and assay efficiencies are given in the present work. The efficacy of these assays for clinical diagnosis of the above-stated stated female reproductive tract disorders (bacterial vaginosis and amnion premature rupture) are discussed.
Putrescine Enzyme, putrescine oxidase Cadaverine Enzyme, diamine oxidase Amperometry Electrode

"The Fluorimetric Determination Of The Lecithin-sphingomyelin Ratio Of Amniotic Fluid After HPLC"
Ann. Clin. Biochem. 1985 Volume 22, Issue 3 Pages 321-323
Gebhardt DO, Soederhuizen W, Feyen JH.

Abstract: Amniotic fluid extracts, in CHCl3 - methanol (2:1), were analyzed by HPLC on a column of Ultrasphere Si (5 µm) with a pre-column of silica gel, with acetonitrile - methanol - water (5:1:1) as mobile phase (1 mL min-1); determination was by post-column derivatization with 1,6-diphenylhexa-1,3,5-triene by incubation for 1 min at 40°C and detection at 460 nm (excitation at 365 nm). The limit of detection for dipalmitoylphosphatidylcholine was 50 nmol. Good correlation (r = 0.99) was obtained between the phosphatidylcholine-to-sphingomyelin ratios obtained fluorimetrically and gravimetrically.
Lecithin Sphingomyelin Clinical analysis HPLC Fluorescence Sample preparation Heated reaction Post-column derivatization