University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological -> deoxyribonucleic acid

Citations 1

"Determination Of Inorganic Phosphate By Flow Injection Method With Immobilized Enzymes And Chemiluminescence Detection"
Anal. Biochem. 1989 Volume 182, Issue 2 Pages 366-370
Hideki Kawasaki, Katsumi Sato, Jyunko Ogawa, Yukio Hasegawa and Hidetaka Yuki

Abstract: Inorganic phosphate (I) was determined by flow injection analysis with use of two glass columns (0.9 cm x 3 mm), one packed with purine-nucleoside phosphorylase-coated controlled-pore glass beads (AMP-500; 120 to 200 mesh), and the second packed with xanthine oxidase and urate oxidase-coated beads (preparation described). The carrier solution (1 mL min-1) was 25 µM inosine in 10 mM HEPES - NaOH buffer (pH 7.5) and the H2O2 produced was determined with use of 10 µM luminol and 0.8 µM peroxidase in 50 mM carbonate buffer (pH 10.5) and chemiluminescence detection. The calibration graph was rectilinear for 5 to 250 pmol of K2HPO4 and the detection limit was 500 fmol. The within-assay coefficient of variation was 1.03 to 9.36%. The method was applied in determination of I in DNA.
Phosphate Chemiluminescence Immobilized enzyme Glass Column Controlled pore glass Buffer