University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Bacteria -> streptomyces chromofuscus

Citations 1

"A New Method To Determine Enzyme Activities In Two-phase Systems By Flow Injection Analysis"
Biosens. Bioelectron. 1998 Volume 13, Issue 7-8 Pages 925-930
M. Becker* and U. Spohn

Abstract: The activity of phospholipase D (PLD) from Streptomyces chromofuscus in two-phase systems consisting of a PLD containing aqueous phase and a phosphatidylcholine (PC) containing organic solvent phase was investigated. Two-phase systems with highly reproducible segmentation were generated in a continuous-flow system using a capillary segmentor. Between 0.01 and 2 U/mL PLD from Streptomyces chromofuscus were detected in a water/hexane two-phase system. The linear detection range was 0.01 to 0.5 U PLD/mL. The apparent KM of Streptomyces chromofuscus PLD in a water/hexane system was 41.43 µM PC, the apparent Vmax was 3.68 µmol min-1 mg-1. The variation of PLD activity in different organic solvents correlated with the solvent's hydrophobicity. If anionic detergents or primary alcohols were added to the two-phase system, concentration dependent activation of the PLD was observed
Enzyme, activity Enzyme, phospholipase D Emulsion