University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Sorghum Malt

Classification: Agricultural -> grain -> sorghum -> malt

Citations 2

"Flow Injection Spectrophotometric Assay Of α-amylase Activity"
Anal. Chim. Acta 2000 Volume 421, Issue 1 Pages 19-25
J. F. van Staden and L. V. Mulaudzi

Abstract: A spectrophotometric flow injection method for the determination of α-amylase activity is proposed. The method is based on the analysis of the maltose obtained during the hydrolysis of amylose in the presence of α-amylase and relies on the reaction of 3,5 dinitrosalicylic-acid and maltose at boiling temperatures to form a brick red colored complex, monitored spectrophotometrically at 540 nm. The method has proved to be highly sensitive and rapid to allow analysis of 60 samples per hour. The % RSD of this method was found to be <0.5% with the working activity range of 0.005-0.06 FAU. The detection limit was found to be 0.0048 FAU. (FAU: fungal amylase unit; 1 FAU is the amount of the enzyme that hydrolyses 5.26 g of starch per hour. 1 FAU=249.85 SIGMA).
α-Amylase Spectrophotometry Indirect Heated reaction Method comparison Reactor

"Aflatoxin Analysis By Reversed-phase HPLC Using Post-column Derivatization For Enhancement Of Fluorescence"
J. Liq. Chromatogr. Relat. Technol. 1986 Volume 9, Issue 1 Pages 103-112
P. G. Thiel; S. Stockenstrom; P. S. Gathercole

Abstract: Aflatoxins extracted from maize, peanut butter, sorghum malt or duckling mash were subjected to HPLC on a column (7.5 cm x 4.6 mm) of Altex Ultrasphere ODS (3 µm) with a mobile phase (1 mL min-1) of 0.01 M KH2PO4 - acetonitrile - methanol (39:9:7); the eluate was treated with saturated aqueous iodine at 60°C before the fluorescence was measured at 440 nm (excitation at 365 nm). The calibration graphs were rectilinear for 1.16, 0.48, 1.48 and 0.84 ng µL-1 for aflatoxins B1, B2, G1 and G2, respectively.
Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 HPLC Fluorescence Heated reaction Post-column derivatization